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To analyze the abundance of proteins associated with metabolism in control (330 mOsm) and HOS (450 mOsm) hTCEpi cells, we immunoblotted for LDHA, PGK1, and MDH2. (A) Immunoblot analysis of isosmolar control and HOS whole-cell lysates showed no difference in LDHA and PGK1 protein levels. MDH2 expression was reduced in HOS hTCEpi cells. (B-D) Quantification of chemiluminescent immunodetection signal density on immunoblots, *p<0.05, t-test, N=3. β-actin was used as a loading control.

Journal: bioRxiv

Article Title: Hyperosmolar stress promotes the release of small extracellular vesicles containing metabolic proteins from corneal epithelial cells

doi: 10.64898/2026.03.27.714594

Figure Lengend Snippet: To analyze the abundance of proteins associated with metabolism in control (330 mOsm) and HOS (450 mOsm) hTCEpi cells, we immunoblotted for LDHA, PGK1, and MDH2. (A) Immunoblot analysis of isosmolar control and HOS whole-cell lysates showed no difference in LDHA and PGK1 protein levels. MDH2 expression was reduced in HOS hTCEpi cells. (B-D) Quantification of chemiluminescent immunodetection signal density on immunoblots, *p<0.05, t-test, N=3. β-actin was used as a loading control.

Article Snippet: Anti-Annexin 2 (Abcam), Anti-Alix (Abcam), Anti-PGK1 (Abcam), Anti-LDH2 (Cell Signaling), Anti-Calreticulin (Cell Signaling), and anti-SOD1 (Proteintech), β-actin rabbit HRP conjugated (Cell Signaling), and α-tubulin (Cell Signaling) were used at 1:1000.

Techniques: Control, Western Blot, Expressing, Immunodetection

To analyze the abundance of proteins associated with adhesion in control (330 mOsm) and HOS (450 mOsm) hTCEpi cells, we immunoblotted for JUP, DSG1, and DSP. (A) Western blot analysis of control and HOS hTCEpi whole-cell lysates showed no difference in DSG1 and DSP protein levels. However, we detected a decrease in JUP levels in HOS CECs. (B-D) Quantification of chemiluminescent immunodetection signal density on immunoblots, **p<0.01, t-test, N=3. β-actin was used as a loading control.

Journal: bioRxiv

Article Title: Hyperosmolar stress promotes the release of small extracellular vesicles containing metabolic proteins from corneal epithelial cells

doi: 10.64898/2026.03.27.714594

Figure Lengend Snippet: To analyze the abundance of proteins associated with adhesion in control (330 mOsm) and HOS (450 mOsm) hTCEpi cells, we immunoblotted for JUP, DSG1, and DSP. (A) Western blot analysis of control and HOS hTCEpi whole-cell lysates showed no difference in DSG1 and DSP protein levels. However, we detected a decrease in JUP levels in HOS CECs. (B-D) Quantification of chemiluminescent immunodetection signal density on immunoblots, **p<0.01, t-test, N=3. β-actin was used as a loading control.

Article Snippet: Anti-Annexin 2 (Abcam), Anti-Alix (Abcam), Anti-PGK1 (Abcam), Anti-LDH2 (Cell Signaling), Anti-Calreticulin (Cell Signaling), and anti-SOD1 (Proteintech), β-actin rabbit HRP conjugated (Cell Signaling), and α-tubulin (Cell Signaling) were used at 1:1000.

Techniques: Control, Western Blot, Immunodetection